Tag

Part:BBa_J70620:Design

Designed by: Joseph Lynch   Group: Knight Lab   (2010-08-05)

RFC12 Maltose Binding Protein Internal Domain [malE]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 55


Design Notes

Primers: 

 GTTTCTT C GAATTC GCGGCCGC T ACTAGT AAAATCGAAGAAGGTAAACTGGTAATCTGG, malE-F
 GTTTCTT C CTGCAG CGGCCGC T GCTAGC GTTGTTGTTATTGTTATTGTTGTTGTTGTTCGAG, malE-RN
 GTTTCTT C CTGCAG CGGCCGC T GCTAGC AGTCTGCGCGTCTTTCAGGGC, malE-R

Source

NEB:[http://www.neb.com/nebecomm/products/productn8076.asp pMAL-c2x]


References

<biblio>

  1. Kimple pmid=18429272
  2. Terpe pmid=12536251

</biblio>